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IHC image of CSB-PA018838LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system.
IHC image of CSB-PA018838LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system.
IHC image of CSB-PA018838LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system.

PSAT1 Antibody

CSB-PA018838LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPSAT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSAT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29968
  • Target name
    PSAT1
  • Target description
    phosphoserine aminotransferase 1
  • Target synonyms
    EPIP, NLS2, PSA, PSAT, PSATD, phosphoserine aminotransferase, endometrial progesterone-induced protein, phosphohydroxythreonine aminotransferase, phosphoserine transaminase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y617
  • Protein Name
    Phosphoserine aminotransferase
  • Scientific Description
    Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PSAT1 using anti-PSAT1 antibody (A06277). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSAT1 antigen affinity purified polyclonal antibody (Catalog # A06277) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSAT1 at approximately 40 kDa. The expected band size for PSAT1 is at 37-45 kDa.
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Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.
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