Anti-PSMA [8C12]

Absolute Antibody

Anti-PSMA [8C12]

9

The antibody is specific for PSMA. The antibody recognises a conformational epitope.

The specificity of the antibody for PSMA was determined by ELISA analysis. The ability of the original format of the antibody and the bispecific 14A8 x 8C12 antibody to bind to PSMA-expressing LNCaP cells and CD89-expressing U937 cells was evaluated by solid phase ELISA. The antibody demonstrated high specificity for LNCaP cell membranes over a range of antigen concentrations, while the bispecific molecule binds both LNCaP and U937 cells in a dose-dependent fashion. The original format of the antibody immunoprecipitated PSMA from LNCaP Cells. This antibody was used for detection of PSMA expressed on LNCaP cells by flow cytometry. The original format and the bispecific 14A8 x 8C12 antibodies were tested for cell- mediated ADCC killing of labeled PSMA-expressing tumor cells. The results showed that both versions mediated dose dependent lysis by monocytes of LNCaP tumor cells. The original format and the bispecific 14A8 x 8C12 were tested for their ability to mediate phagocytosis of labeled PSMA-expressing tumor cells (LNCaP cells). Results showed that both were able to mediate phagocytosis of LNCaP cells in a dose dependent fashion (WO2003064606A3).

Flow Cytometry, ImmunoPrecipitation, ELISA, Other Application

Research Use Only

Monoclonal

8C12

FOLH1

folate hydrolase 1

cell growth-inhibiting gene 27 protein; FGCP; FOLH; folylpoly-gamma-glutamate carboxypeptidase; GCP2; GCPII; glutamate carboxylase II; glutamate carboxypeptidase 2; glutamate carboxypeptidase II; membrane glutamate carboxypeptidase; mGCP; NAALAD1; NAALADase I; N-acetylated alpha-linked acidic dipeptidase 1; N-acetylated-alpha-linked acidic dipeptidase I; prostate specific membrane antigen variant F; PSM; PSMA; pteroylpoly-gamma-glutamate carboxypeptidase

Rabbit

IgG

Glutamate carboxypeptidase 2

Human

-20°C,2°C to 8°C

12352203