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Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.

Anti-PSMC3 Antibody

HPA006065
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPSMC3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PSMC3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5702
  • Target name
    PSMC3
  • Target description
    proteasome 26S subunit, ATPase 3
  • Target synonyms
    DCIDP, RPT5, TBP1, 26S proteasome regulatory subunit 6A, 26S protease regulatory subunit 6A, 26S proteasome AAA-ATPase subunit RPT5, Tat-binding protein 1, human immunodeficiency virus tat transactivator binding protein-1, proteasome (prosome, macropain) 26S subunit, ATPase, 3, proteasome subunit P50, testicular secretory protein Li 42
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17980
  • Protein Name
    26S proteasome regulatory subunit 6A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (A07208-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human U87 whole cell lysates, Lane 8: rat stomach tissue lysates, Lane 9: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBP-1/PSMC3 antigen affinity purified polyclonal antibody (Catalog # A07208-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBP-1/PSMC3 at approximately 50KD. The expected band size for TBP-1/PSMC3 is at 50KD.
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