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Figure 1. Western blot analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (A07208-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human U87 whole cell lysates, Lane 8: rat stomach tissue lysates, Lane 9: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBP-1/PSMC3 antigen affinity purified polyclonal antibody (Catalog # A07208-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBP-1/PSMC3 at approximately 50KD. The expected band size for TBP-1/PSMC3 is at 50KD.
Figure 1. Western blot analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (A07208-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human U87 whole cell lysates, Lane 8: rat stomach tissue lysates, Lane 9: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBP-1/PSMC3 antigen affinity purified polyclonal antibody (Catalog # A07208-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBP-1/PSMC3 at approximately 50KD. The expected band size for TBP-1/PSMC3 is at 50KD.
Figure 1. Western blot analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (A07208-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human U87 whole cell lysates, Lane 8: rat stomach tissue lysates, Lane 9: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBP-1/PSMC3 antigen affinity purified polyclonal antibody (Catalog # A07208-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBP-1/PSMC3 at approximately 50KD. The expected band size for TBP-1/PSMC3 is at 50KD.

Anti-TBP-1/PSMC3 Antibody Picoband(r)

A07208-1-CARRIER-FREE
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPSMC3
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-TBP-1/PSMC3 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID5702
  • Target name
    PSMC3
  • Target description
    proteasome 26S subunit, ATPase 3
  • Target synonyms
    26S protease regulatory subunit 6A; 26S proteasome AAA-ATPase subunit RPT5; 26S proteasome regulatory subunit 6A; DCIDP; human immunodeficiency virus tat transactivator binding protein-1; proteasome (prosome, macropain) 26S subunit, ATPase, 3; proteasome subunit P50; RPT5; Tat-binding protein 1; TBP1; testicular secretory protein Li 42
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17980
  • Protein Name
    26S proteasome regulatory subunit 6A
  • Scientific Description
    Boster Bio Anti-TBP-1/PSMC3 Antibody Picoband® catalog # A07208-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203