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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMC3 Polyclonal Antibody, Unconjugated (bs-19463R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMC3 Polyclonal Antibody, Unconjugated (bs-19463R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMC3 Polyclonal Antibody, Unconjugated (bs-19463R) at 1:400 overnight at 4°C, DAB staining.

PSMC3 Polyclonal Antibody

BS-19463R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPSMC3
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Overview

  • Supplier
    Bioss
  • Product Name
    PSMC3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5702
  • Target name
    PSMC3
  • Target description
    proteasome 26S subunit, ATPase 3
  • Target synonyms
    DCIDP, RPT5, TBP1, 26S proteasome regulatory subunit 6A, 26S protease regulatory subunit 6A, 26S proteasome AAA-ATPase subunit RPT5, Tat-binding protein 1, human immunodeficiency virus tat transactivator binding protein-1, proteasome (prosome, macropain) 26S subunit, ATPase, 3, proteasome subunit P50, testicular secretory protein Li 42
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17980
  • Protein Name
    26S proteasome regulatory subunit 6A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (A07208-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human U87 whole cell lysates, Lane 8: rat stomach tissue lysates, Lane 9: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBP-1/PSMC3 antigen affinity purified polyclonal antibody (Catalog # A07208-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBP-1/PSMC3 at approximately 50KD. The expected band size for TBP-1/PSMC3 is at 50KD.
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