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Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Anti-PTGES2 Antibody

HPA020733
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPTGES2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PTGES2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80142
  • Target name
    PTGES2
  • Target description
    prostaglandin E synthase 2
  • Target synonyms
    C9orf15, GBF-1, GBF1, PGES2, mPGES-2, prostaglandin E synthase 2, GATE-binding factor 1, gamma-interferon-activated transcriptional element-binding factor 1, mPGE synthase-2, membrane-associated prostaglandin E synthase 2, microsomal prostaglandin E synthase-2, prostaglandin-H(2) E-isomerase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H7Z7
  • Protein Name
    Prostaglandin E synthase 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PTGES2/Gbf1 using anti-PTGES2/Gbf1 antibody (A06706-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGES2/Gbf1 antigen affinity purified polyclonal antibody (Catalog # A06706-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGES2/Gbf1 at approximately 42 kDa. The expected band size for PTGES2/Gbf1 is at 42 kDa.
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