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PTGES2 Antibody

LS-C748491
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetPTGES2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PTGES2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 41kDa, while the observed MW by Western blot was 34kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 41kDa, while the observed MW by Western blot was 34kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID80142
  • Target name
    PTGES2
  • Target description
    prostaglandin E synthase 2
  • Target synonyms
    C9orf15, GBF-1, GBF1, PGES2, mPGES-2, prostaglandin E synthase 2, GATE-binding factor 1, gamma-interferon-activated transcriptional element-binding factor 1, mPGE synthase-2, membrane-associated prostaglandin E synthase 2, microsomal prostaglandin E synthase-2, prostaglandin-H(2) E-isomerase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTGES2/Gbf1 using anti-PTGES2/Gbf1 antibody (A06706-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGES2/Gbf1 antigen affinity purified polyclonal antibody (Catalog # A06706-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGES2/Gbf1 at approximately 42 kDa. The expected band size for PTGES2/Gbf1 is at 42 kDa.
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Western blot All lanes: PTGES2 antibody at 3.77microg/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
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