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Immunofluorescent staining of human cell line AF22 shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line AF22 shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line AF22 shows localization to endoplasmic reticulum.

Anti-PTN Antibody

HPA073913
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPTN
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PTN
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5764
  • Target name
    PTN
  • Target description
    pleiotrophin
  • Target synonyms
    HARP; HBBM; HB-GAM; HBGF8; HBGF-8; HBNF; HBNF-1; heparin affin regulatory protein; heparin-binding brain mitogen; heparin-binding growth factor 8; heparin-binding growth-associated molecule; heparin-binding neurite outgrowth promoting factor; heparin-binding neurite outgrowth-promoting factor 1; NEGF1; OSF-1; osteoblast-specific factor 1; pleiotrophin; pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21246
  • Protein Name
    Pleiotrophin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Pleiotrophin using anti-Pleiotrophin antibody (A01368-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pleiotrophin antigen affinity purified polyclonal antibody (Catalog # A01368-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pleiotrophin at approximately 19KD. The expected band size for Pleiotrophin is at 19KD.
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