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Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Anti-RAB11A Antibody

HPA060686
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRAB11A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RAB11A
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8766
  • Target name
    RAB11A
  • Target description
    RAB11A, member RAS oncogene family
  • Target synonyms
    YL8, ras-related protein Rab-11A, RAB 11A, member oncogene family, rab-11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62491
  • Protein Name
    Ras-related protein Rab-11A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Rab11A using anti-Rab11A antibody (PB9789). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human 22RV1 whole cell lysates, Lane 6: human SGC-7901 whole cell lysates, Lane 7: human MDA-MB-453 whole cell lysates, Lane 8: human Jurkat whole cell lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: rat brain tissue lysates, Lane 12: mouse testis tissue lysates, Lane 13: mouse brain tissue lysates, Lane 14: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab11A antigen affinity purified polyclonal antibody (Catalog # PB9789) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab11A at approximately 24 kDa. The expected band size for Rab11A is at 24 kDa.
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