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Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Anti-RAB1A Antibody

HPA056141
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRAB1A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RAB1A
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5861
  • Target name
    RAB1A
  • Target description
    RAB1A, member RAS oncogene family
  • Target synonyms
    GTP binding protein Rab1a; Rab GTPase YPT1 homolog; RAB1; RAB1, member RAS oncogene family; ras-related protein Rab-1A; YPT1; YPT1-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62820
  • Protein Name
    Ras-related protein Rab-1A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: RAB1A antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 23, 16, 14 kDa Observed band size: 80 kDa
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Figure 1. Western blot analysis of RAB1A using anti-RAB1A antibody (A02682-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB1A antigen affinity purified polyclonal antibody (Catalog # A02682-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB1A at approximately 23,22 kDa. The expected band size for RAB1A is at 23 kDa.
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