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Rab1A Polyclonal Antibody

CAC07341
Biomatik
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetRAB1A
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Overview

  • Supplier
    Biomatik
  • Product Name
    Rab1A Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB; Recommended dilution: WB:1:1000-1:5000
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5861
  • Target name
    RAB1A
  • Target description
    RAB1A, member RAS oncogene family
  • Target synonyms
    RAB1, YPT1, ras-related protein Rab-1A, GTP binding protein Rab1a, RAB1, member RAS oncogene family, Rab GTPase YPT1 homolog, YPT1-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62820
  • Protein Name
    Ras-related protein Rab-1A
  • Scientific Description
    The Rab1A Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAB1A using anti-RAB1A antibody (A02682-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB1A antigen affinity purified polyclonal antibody (Catalog # A02682-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB1A at approximately 23,22 kDa. The expected band size for RAB1A is at 23 kDa.
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Western blot All lanes: RAB1A antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 23, 16, 14 kDa Observed band size: 80 kDa
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Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Rab 1A antibody [N1C3] (GTX101454) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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