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Rab 1A antibody [N1C3]

GTX101454
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetRAB1A
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Overview

  • Supplier
    GeneTex
  • Product Name
    Rab 1A antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.26 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5861
  • Target name
    RAB1A
  • Target description
    RAB1A, member RAS oncogene family
  • Target synonyms
    GTP binding protein Rab1a; Rab GTPase YPT1 homolog; RAB1; RAB1, member RAS oncogene family; ras-related protein Rab-1A; YPT1; YPT1-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62820
  • Protein Name
    Ras-related protein Rab-1A
  • Scientific Description
    This gene encodes a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Subcellular Distribution of BALF2 and the Role of Rab1 in the Formation of Epstein-Barr Virus Cytoplasmic Assembly Compartment and Virion Release.
    Read more

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Figure 1. Western blot analysis of RAB1A using anti-RAB1A antibody (A02682-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB1A antigen affinity purified polyclonal antibody (Catalog # A02682-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB1A at approximately 23,22 kDa. The expected band size for RAB1A is at 23 kDa.
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