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Rab6-GTP is detected by immunocytochemistry using anti-Rab6-GTP, mAb (AA2) (Prod. No. AG-27B-0004). Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Sap
Rab6-GTP is detected by immunocytochemistry using anti-Rab6-GTP, mAb (AA2) (Prod. No. AG-27B-0004). Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Sap
Rab6-GTP is detected by immunocytochemistry using anti-Rab6-GTP, mAb (AA2) (Prod. No. AG-27B-0004). Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Sap

anti-Rab6-GTP, mAb (rec.) (AA2)

AG-27B-0004
AdipoGen Life Sciences
ApplicationsImmunoCytoChemistry
Product group Antibodies
TargetRAB6A
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-Rab6-GTP, mAb (rec.) (AA2)
  • Delivery Days Customer
    10
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AA2
  • Concentration
    1 mg/ml
  • Estimated Purity
    >95%
  • Gene ID5870
  • Target name
    RAB6A
  • Target description
    RAB6A, member RAS oncogene family
  • Target synonyms
    Rab GTPase; RAB6; RAB6, member RAS oncogene family; ras-related protein Rab-6A
  • Host
    Human
  • Isotype
    IgG2
  • Protein IDP20340
  • Protein Name
    Ras-related protein Rab-6A
  • Scientific Description
    Rab6 is involved in protein transport. It is a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). - Recombinant Antibody. Recognizes human, mouse and Drosophila GTP-bound Rab6a and Rab6b and mutant Rab6Q72L. Does not detect Rab6 GDP. Species cross-reactivity: Drosophila, Human, Mouse. Clone: AA2. Isotype: Human IgG2lambda. Applications: ICC. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. Rab6 is involved in protein transport. It is a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER).
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAB6A using anti-RAB6A antibody (A02911-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: Human A431 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB6A antigen affinity purified polyclonal antibody (Catalog # A02911-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB6A at approximately 24KD. The expected band size for RAB6A is at 24KD.
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