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RAB6A antibody

GTX110646
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRAB6A
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Overview

  • Supplier
    GeneTex
  • Product Name
    RAB6A antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.21 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5870
  • Target name
    RAB6A
  • Target description
    RAB6A, member RAS oncogene family
  • Target synonyms
    RAB6, ras-related protein Rab-6A, RAB6, member RAS oncogene family, Rab GTPase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20340
  • Protein Name
    Ras-related protein Rab-6A
  • Scientific Description
    Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Kawabata M, Matsuo H, Koito T, et al. Legionella hijacks the host Golgi-to-ER retrograde pathway for the association of Legionella-containing vacuole with the ER. PLoS Pathog. 2021,17(3):e1009437. doi: 10.1371/journal.ppat.1009437
    Read this paper
  • Ishida M, Bonifacino JS. ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network. J Cell Biol. 2019,218(11):3681-3696. doi: 10.1083/jcb.201905097
    Read this paper
  • Wu CH, Hsiao YM, Yeh KT, et al. Upregulation of microRNA-4417 and Its Target Genes Contribute to Nickel Chloride-promoted Lung Epithelial Cell Fibrogenesis and Tumorigenesis. Sci Rep. 2017,7(1):15320. doi: 10.1038/s41598-017-14610-7
    Read this paper
  • Lee PL, Ohlson MB, Pfeffer SR. Rab6 regulation of the kinesin family KIF1C motor domain contributes to Golgi tethering. Elife. 2015,4. doi: 10.7554/eLife.06029
    Read this paper

Datasheet

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Figure 1. Western blot analysis of RAB6A using anti-RAB6A antibody (A02911-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: Human A431 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB6A antigen affinity purified polyclonal antibody (Catalog # A02911-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB6A at approximately 24KD. The expected band size for RAB6A is at 24KD.
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