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Immunohistochemical staining of human Small intestine shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human Small intestine shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human Small intestine shows strong granular cytoplasmic positivity in glandular cells.

Anti-RAB6A Antibody

HPA059131
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRAB6A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RAB6A
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5870
  • Target name
    RAB6A
  • Target description
    RAB6A, member RAS oncogene family
  • Target synonyms
    Rab GTPase; RAB6; RAB6, member RAS oncogene family; ras-related protein Rab-6A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20340
  • Protein Name
    Ras-related protein Rab-6A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAB6A using anti-RAB6A antibody (A02911-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: Human A431 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB6A antigen affinity purified polyclonal antibody (Catalog # A02911-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB6A at approximately 24KD. The expected band size for RAB6A is at 24KD.
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Rab6-GTP is detected by immunocytochemistry using anti-Rab6-GTP, mAb (AA2) (Prod. No. AG-27B-0004). Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Sap
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