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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Anti-RAD21 Antibody

HPA020044
Atlas Antibodies
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRAD21
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RAD21 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5885
  • Target name
    RAD21
  • Target description
    RAD21 cohesin complex component
  • Target synonyms
    CDLS4, HR21, HRAD21, MCD1, MGS, NXP1, SCC1, hHR21, double-strand-break repair protein rad21 homolog, NXP-1, RAD21 homolog, SCC1 homolog, kleisin, nuclear matrix protein 1, protein involved in DNA double-strand break repair, sister chromatid cohesion 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60216
  • Protein Name
    Double-strand-break repair protein rad21 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of RAD21 using anti-RAD21 antibody (A01864-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: human T-47D whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: rat pancreas tissue lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD21 antigen affinity purified polyclonal antibody (Catalog # A01864-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD21 at approximately 72 kDa. The expected band size for RAD21 is at 72 kDa.
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