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Rad21 Antibody (YA2985, Rabbit)

HY-P83240
MedChem Express
TargetRAD21
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Rad21 Antibody (YA2985, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5885
  • Target name
    RAD21
  • Target description
    RAD21 cohesin complex component
  • Target synonyms
    CDLS4, HR21, HRAD21, MCD1, MGS, NXP1, SCC1, hHR21, double-strand-break repair protein rad21 homolog, NXP-1, RAD21 homolog, SCC1 homolog, kleisin, nuclear matrix protein 1, protein involved in DNA double-strand break repair, sister chromatid cohesion 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rad21 Antibody (YA2985) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2985), targeting Rad21, with a predicted molecular weight of 72 kDa (observed band size: 72,128 kDa). Rad21 Antibody (YA2985) can be used for WB, FC experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RAD21 using anti-RAD21 antibody (A01864-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: human T-47D whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: rat pancreas tissue lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD21 antigen affinity purified polyclonal antibody (Catalog # A01864-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD21 at approximately 72 kDa. The expected band size for RAD21 is at 72 kDa.
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