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Anti-Rad51 [3C10]

AB01312-1.1-BT
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetRAD51
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Rad51 [3C10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: Anti-Rad51 3C10 antibody was used to visualise Rad51 via Western blot after previous immunoprecipitation with different antibodies in experiments analysing the interactions between Rad51 and DMC1 (Masson et al., 1999). IF: 3C10 antibody was used to visualise Rad51 on chromatin in endopolyploid tumour cells in experiments investigating the role of another protein - REC8 in chromosome segregation after radiation (Erenpreisa et al., 2009). IHC: This antibody was also used to immunohistochemically stain biopsy and resected specimens of esophagus and squamous cell carcinoma of the esophagus (Nakanoko et al., 2014).
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3C10
  • Gene ID5888
  • Target name
    RAD51
  • Target description
    RAD51 recombinase
  • Target synonyms
    BRCA1/BRCA2-containing complex, subunit 5; BRCC5; DNA repair protein RAD51 homolog 1; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51 homolog A; RAD51A; RECA; RecA, E. coli, homolog of; RecA-like protein; recombination protein A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ06609
  • Protein Name
    DNA repair protein RAD51 homolog 1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Rad51 using anti-Rad51 antibody (A00088). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human A431 whole cell lysate, Lane 3: human 293T whole cell lysate, Lane 4: human K562 whole cell lysate, Lane 5: human Jurkat whole cell lysate, Lane 6: human A549 whole cell lysate, Lane 7: human Caco-2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody (Catalog # A00088) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rad51 at approximately 39KD. The expected band size for Rad51 is at 36KD.
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