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RAD51 Polyclonal Antibody

CAC14547
Biomatik
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRAD51
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Overview

  • Supplier
    Biomatik
  • Product Name
    RAD51 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5888
  • Target name
    RAD51
  • Target description
    RAD51 recombinase
  • Target synonyms
    BRCA1/BRCA2-containing complex, subunit 5; BRCC5; DNA repair protein RAD51 homolog 1; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51 homolog A; RAD51A; RECA; RecA, E. coli, homolog of; RecA-like protein; recombination protein A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06609
  • Protein Name
    DNA repair protein RAD51 homolog 1
  • Scientific Description
    The RAD51 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF, IP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Rad51 using anti-Rad51 antibody (A00088). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human A431 whole cell lysate, Lane 3: human 293T whole cell lysate, Lane 4: human K562 whole cell lysate, Lane 5: human Jurkat whole cell lysate, Lane 6: human A549 whole cell lysate, Lane 7: human Caco-2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody (Catalog # A00088) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rad51 at approximately 39KD. The expected band size for Rad51 is at 36KD.
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