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ProductsBack/Anti-Rad51C [2H11/6]

Anti-Rad51C [2H11/6]

Ab01304-1.1
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetRAD51C
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Rad51C [2H11/6]
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: Rad51C 2H11/6 antibody was used to visualise Rad51C on Western blots in multiple experiments (Masson et al., 2001). IF/ICC: This antibody was also used to stain Rad51C in HEK293 cells during experiments aimed at determining the cellular localization of Rad51C (Bennett and Knight, 2005).
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; IP; IF; IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2H11/6
  • Gene ID5889
  • Target name
    RAD51C
  • Target description
    RAD51 paralog C
  • Target synonyms
    BROVCA3; DNA repair protein RAD51 homolog 3; FANCO; R51H3; RAD51L2; RAD51-like protein 2; yeast RAD51 homolog 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO43502
  • Protein Name
    DNA repair protein RAD51 homolog 3
  • Reactivity Supplier
    Human, Mouse, Primate, Yeast
  • Reactivity Supplier Note
    This antibody was raised by immunising mice with the denaturated recombinant human RAD51C-His10 protein which had been purified from 2.4 liters of E. coli FB810 recA− pLysS carrying pRAD51C-16b.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAD51C using anti-RAD51C antibody (A01837-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD51C antigen affinity purified polyclonal antibody (Catalog # A01837-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD51C at approximately 42 kDa. The expected band size for RAD51C is at 42 kDa.
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