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Western Blot analysis of COS7 cells using Rad51C Polyclonal Antibody
Western Blot analysis of COS7 cells using Rad51C Polyclonal Antibody
Western Blot analysis of COS7 cells using Rad51C Polyclonal Antibody

RAD51C Antibody

CSB-PA003917
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRAD51C
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAD51C Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5889
  • Target name
    RAD51C
  • Target description
    RAD51 paralog C
  • Target synonyms
    BROVCA3; DNA repair protein RAD51 homolog 3; FANCO; R51H3; RAD51L2; RAD51-like protein 2; yeast RAD51 homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43502
  • Protein Name
    DNA repair protein RAD51 homolog 3
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAD51C using anti-RAD51C antibody (A01837-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD51C antigen affinity purified polyclonal antibody (Catalog # A01837-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD51C at approximately 42 kDa. The expected band size for RAD51C is at 42 kDa.
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