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Anti-Rad51D [5A8/4]

Ab01307-1.1
Absolute Antibody
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetRAD51D
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Rad51D [5A8/4]
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: Rad51D 5A8/4 (5A8) antibody detected Rad51D in a material from V79 and irs3 cells in Western blot analysis of immunoprecipitated Rad51 complexes (French et al., 2002). WB: This antibody was also applied in experiments studying the role of Rad51D in the DNA repair where the antibody detected Rad51D protein on Western blots from CHO AA8 cells (Hinz et al., 2006).
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Applications Supplier
    WB; IP; ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5A8/4
  • Gene ID5892
  • Target name
    RAD51D
  • Target description
    RAD51 paralog D
  • Target synonyms
    BROVCA4; DNA repair protein RAD51 homolog 4; R51H3; RAD51 homolog D; RAD51L3; RAD51-like protein 3; recombination repair protein; TRAD
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO75771
  • Protein Name
    DNA repair protein RAD51 homolog 4
  • Reactivity Supplier
    Human, Hamster
  • Reactivity Supplier Note
    This antibody was raised by immunising mice with the denaturated recombinant human Rad51D protein which had been previously amplified and purified from E. coli.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAD51D using anti-RAD51D antibody (A02893-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A2780 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD51D antigen affinity purified polyclonal antibody (Catalog # A02893-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD51D at approximately 30-40 kDa. The expected band size for RAD51D is at 35 kDa.
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