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Rad51D antibody

GTX11051
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetRAD51D
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Overview

  • Supplier
    GeneTex
  • Product Name
    Rad51D antibody [Rad51D 5A8/4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: Use at an assay dependent dilution. Predicted molecular weight: 39 kDa. Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID5892
  • Target name
    RAD51D
  • Target description
    RAD51 paralog D
  • Target synonyms
    BROVCA4; DNA repair protein RAD51 homolog 4; R51H3; RAD51 homolog D; RAD51L3; RAD51-like protein 3; recombination repair protein; TRAD
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO75771
  • Protein Name
    DNA repair protein RAD51 homolog 4
  • Scientific Description
    The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream ring finger and FYVE-like domain containing 1 (RFFL) gene. [provided by RefSeq, Jan 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability. French CA et al., 2002 May 31, J Biol Chem
    Read more

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Figure 1. Western blot analysis of RAD51D using anti-RAD51D antibody (A02893-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A2780 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD51D antigen affinity purified polyclonal antibody (Catalog # A02893-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD51D at approximately 30-40 kDa. The expected band size for RAD51D is at 35 kDa.
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