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ProductsBack/RAD51D Antibody
Western blot All lanes: DNA repair protein RAD51 homolog 4 antibody at 8ug/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36, 6, 24, 31, 13, 23, 38 kDa Observed band size: 36 kDa
Western blot All lanes: DNA repair protein RAD51 homolog 4 antibody at 8ug/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36, 6, 24, 31, 13, 23, 38 kDa Observed band size: 36 kDa
Western blot All lanes: DNA repair protein RAD51 homolog 4 antibody at 8ug/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36, 6, 24, 31, 13, 23, 38 kDa Observed band size: 36 kDa

RAD51D Antibody

CSB-PA019268ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRAD51D
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAD51D Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5892
  • Target name
    RAD51D
  • Target description
    RAD51 paralog D
  • Target synonyms
    BROVCA4; DNA repair protein RAD51 homolog 4; R51H3; RAD51 homolog D; RAD51L3; RAD51-like protein 3; recombination repair protein; TRAD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75771
  • Protein Name
    DNA repair protein RAD51 homolog 4
  • Scientific Description
    Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAD51D using anti-RAD51D antibody (A02893-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A2780 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD51D antigen affinity purified polyclonal antibody (Catalog # A02893-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD51D at approximately 30-40 kDa. The expected band size for RAD51D is at 35 kDa.
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