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Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts, Leydig cells exhibited strong cytoplasmic staining.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts, Leydig cells exhibited strong cytoplasmic staining.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts, Leydig cells exhibited strong cytoplasmic staining.

Anti-RANBP9 Antibody

HPA050007
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRANBP9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RANBP9
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10048
  • Target name
    RANBP9
  • Target description
    RAN binding protein 9
  • Target synonyms
    BPM-L, BPM90, RANBPM, RanBP7, ran-binding protein 9, Ran Binding Protein in the Microtubule organizing center, novel centrosomal protein RanBPM, ran binding protein, centrosomal, ran-binding protein M
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96S59
  • Protein Name
    Ran-binding protein 9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Western Blot Positive WB detected in: A549 whole cell lysate, Raji whole cell lysate, HL60 whole cell lysate, U251 whole cell lysate All lanes: RANBP9 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 78, 44, 56 kDa Observed band size: 78 kDa
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Figure 1. Western blot analysis of RANBP9 using anti-RANBP9 antibody (A03448-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANBP9 antigen affinity purified polyclonal antibody (Catalog # A03448-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANBP9 at approximately 91 kDa. The expected band size for RANBP9 is at 78 kDa.
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