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RANBPM Antibody

LS-C747286
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetRANBP9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RANBPM Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 43kDa/55kDa/77kDa, while the observed MW by Western blot was 100kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) The predicted MW is 43kDa/55kDa/77kDa, while the observed MW by Western blot was 100kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10048
  • Target name
    RANBP9
  • Target description
    RAN binding protein 9
  • Target synonyms
    BPM-L, BPM90, RANBPM, RanBP7, ran-binding protein 9, Ran Binding Protein in the Microtubule organizing center, novel centrosomal protein RanBPM, ran binding protein, centrosomal, ran-binding protein M
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: A549 whole cell lysate, Raji whole cell lysate, HL60 whole cell lysate, U251 whole cell lysate All lanes: RANBP9 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 78, 44, 56 kDa Observed band size: 78 kDa
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Figure 1. Western blot analysis of RANBP9 using anti-RANBP9 antibody (A03448-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANBP9 antigen affinity purified polyclonal antibody (Catalog # A03448-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANBP9 at approximately 91 kDa. The expected band size for RANBP9 is at 78 kDa.
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