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Western Blot Positive WB detected in: A549 whole cell lysate, Raji whole cell lysate, HL60 whole cell lysate, U251 whole cell lysate All lanes: RANBP9 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 78, 44, 56 kDa Observed band size: 78 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, Raji whole cell lysate, HL60 whole cell lysate, U251 whole cell lysate All lanes: RANBP9 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 78, 44, 56 kDa Observed band size: 78 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, Raji whole cell lysate, HL60 whole cell lysate, U251 whole cell lysate All lanes: RANBP9 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 78, 44, 56 kDa Observed band size: 78 kDa

RANBP9 Antibody

CSB-PA853501OA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRANBP9
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Overview

  • Supplier
    Cusabio
  • Product Name
    RANBP9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10048
  • Target name
    RANBP9
  • Target description
    RAN binding protein 9
  • Target synonyms
    BPM90; BPM-L; novel centrosomal protein RanBPM; Ran Binding Protein in the Microtubule organizing center; ran binding protein, centrosomal; ran-binding protein 9; ran-binding protein M; RanBP7; RANBPM
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96S59
  • Protein Name
    Ran-binding protein 9
  • Scientific Description
    May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RANBP9 using anti-RANBP9 antibody (A03448-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANBP9 antigen affinity purified polyclonal antibody (Catalog # A03448-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANBP9 at approximately 91 kDa. The expected band size for RANBP9 is at 78 kDa.
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