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Immunohistochemical staining of human testis shows strong nuclear positivity in subset of cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in subset of cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in subset of cells in seminiferous ducts.

Anti-REC8 Antibody

HPA031727
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetREC8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-REC8
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9985
  • Target name
    REC8
  • Target description
    REC8 meiotic recombination protein
  • Target synonyms
    cohesin Rec8p; cohesion rec8p; HR21spB; human homolog of rad21, S. pombe; kleisin-alpha; meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family; meiotic recombination protein REC8 homolog; meiotic recombination protein REC8-like 1; REC8 homolog; REC8L1; REC8-like 1; Rec8p; recombination and sister chromatid cohesion protein homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95072
  • Protein Name
    Meiotic recombination protein REC8 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western blot All lanes: REC8 antibody at 1.67microg/ml Lane 1: Jurkat whole cell lysate Lane 2: HT29 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 63, 61 kDa Observed band size: 63 kDa
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Figure 1. Western blot analysis of REC8 using anti-REC8 antibody (A04915-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REC8 antigen affinity purified polyclonal antibody (Catalog # A04915-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REC8 at approximately 63KD. The expected band size for REC8 is at 63KD.
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