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Western blot All lanes: REC8 antibody at 1.67microg/ml Lane 1: Jurkat whole cell lysate Lane 2: HT29 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 63, 61 kDa Observed band size: 63 kDa
Western blot All lanes: REC8 antibody at 1.67microg/ml Lane 1: Jurkat whole cell lysate Lane 2: HT29 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 63, 61 kDa Observed band size: 63 kDa
Western blot All lanes: REC8 antibody at 1.67microg/ml Lane 1: Jurkat whole cell lysate Lane 2: HT29 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 63, 61 kDa Observed band size: 63 kDa

REC8 Antibody

CSB-PA019535ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetREC8
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Overview

  • Supplier
    Cusabio
  • Product Name
    REC8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9985
  • Target name
    REC8
  • Target description
    REC8 meiotic recombination protein
  • Target synonyms
    cohesin Rec8p; cohesion rec8p; HR21spB; human homolog of rad21, S. pombe; kleisin-alpha; meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family; meiotic recombination protein REC8 homolog; meiotic recombination protein REC8-like 1; REC8 homolog; REC8L1; REC8-like 1; Rec8p; recombination and sister chromatid cohesion protein homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95072
  • Protein Name
    Meiotic recombination protein REC8 homolog
  • Scientific Description
    Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of REC8 using anti-REC8 antibody (A04915-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REC8 antigen affinity purified polyclonal antibody (Catalog # A04915-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REC8 at approximately 63KD. The expected band size for REC8 is at 63KD.
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