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HT-29 cell lysates probed with REC8 Polyclonal Antibody, Unconjugated (bs-7723R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HT-29 cell lysates probed with REC8 Polyclonal Antibody, Unconjugated (bs-7723R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HT-29 cell lysates probed with REC8 Polyclonal Antibody, Unconjugated (bs-7723R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

REC8 Polyclonal Antibody

BS-7723R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetREC8
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Overview

  • Supplier
    Bioss
  • Product Name
    REC8 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9985
  • Target name
    REC8
  • Target description
    REC8 meiotic recombination protein
  • Target synonyms
    HR21spB, REC8L1, Rec8p, meiotic recombination protein REC8 homolog, REC8 homolog, REC8-like 1, cohesin Rec8p, cohesion rec8p, human homolog of rad21, S. pombe, kleisin-alpha, meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family, meiotic recombination protein REC8-like 1, recombination and sister chromatid cohesion protein homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95072
  • Protein Name
    Meiotic recombination protein REC8 homolog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes. Shimoi G et al., 2019 Feb 8, J Reprod Dev
    Read more

Datasheet

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Figure 1. Western blot analysis of REC8 using anti-REC8 antibody (A04915-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REC8 antigen affinity purified polyclonal antibody (Catalog # A04915-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REC8 at approximately 63KD. The expected band size for REC8 is at 63KD.
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