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Immunohistochemical staining of human adrenal gland shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong membranous positivity in glandular cells.

Anti-RET Antibody

HPA008356
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetRET
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RET
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5979
  • Target name
    RET
  • Target description
    ret proto-oncogene
  • Target synonyms
    cadherin family member 12; cadherin-related family member 16; CDHF12; CDHR16; HSCR1; MEN2A; MEN2B; MTC1; proto-oncogene c-Ret; proto-oncogene tyrosine-protein kinase receptor Ret; PTC; rearranged during transfection; ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease); RET receptor tyrosine kinase; RET-ELE1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07949
  • Protein Name
    Proto-oncogene tyrosine-protein kinase receptor Ret
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RET using anti-RET antibody (A00293-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates Lane 2: human HL-60 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RET antigen affinity purified polyclonal antibody (Catalog # A00293-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RET at approximately 170KD. The expected band size for RET is at 124KD.
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