More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/RET Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RET Polyclonal Antibody, Unconjugated (bs-2793R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RET Polyclonal Antibody, Unconjugated (bs-2793R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RET Polyclonal Antibody, Unconjugated (bs-2793R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

RET Polyclonal Antibody

BS-2793R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRET
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    RET Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5979
  • Target name
    RET
  • Target description
    ret proto-oncogene
  • Target synonyms
    CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET-ELE1, proto-oncogene tyrosine-protein kinase receptor Ret, RET receptor tyrosine kinase, cadherin family member 12, cadherin-related family member 16, proto-oncogene c-Ret, rearranged during transfection, ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07949
  • Protein Name
    Proto-oncogene tyrosine-protein kinase receptor Ret
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of various cells using Ret Polyclonal Antibody
Product group Antibodies
Cusabio
RET AntibodyCSB-PA003961
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRET
  • SizePrice
Product group Antibodies
Biomatik
Ret Recombinant AntibodyCAC12550
ApplicationsFlow Cytometry, ELISA
TargetRET
  • SizePrice
Product group Antibodies
Assay Genie
Ret Polyclonal AntibodyCAB0018
TargetRET
  • SizePrice
Product group Antibodies
MedChem Express
Ret Antibody (YA097, Rabbit)HY-P80307
TargetRET
  • SizePrice
Product group Antibodies
GeneTex
Ret antibodyGTX101059
ApplicationsWestern Blot
TargetRET
  • SizePrice
Product group Antibodies
RayBiotech
Anti-RET Antibody101-11437
TargetRET
  • SizePrice
Figure 1. Western blot analysis of RET using anti-RET antibody (A00293-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates Lane 2: human HL-60 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RET antigen affinity purified polyclonal antibody (Catalog # A00293-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RET at approximately 170KD. The expected band size for RET is at 124KD.
Product group Antibodies
Boster Bio
Anti-RET Antibody Picoband(r)A00293-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetRET
  • SizePrice
Product group Antibodies
Biosynth
Ret antibody70R-RG002
  • SizePrice
Anti-RET Antibody
Product group Antibodies
Antibodies.com
Anti-RET AntibodyA100044
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
RET AntibodyLS-C761146
ApplicationsWestern Blot
TargetRET
  • SizePrice