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RET Antibody

LS-C761146
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetRET
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RET Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5979
  • Target name
    RET
  • Target description
    ret proto-oncogene
  • Target synonyms
    cadherin family member 12; cadherin-related family member 16; CDHF12; CDHR16; HSCR1; MEN2A; MEN2B; MTC1; proto-oncogene c-Ret; proto-oncogene tyrosine-protein kinase receptor Ret; PTC; rearranged during transfection; ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease); RET receptor tyrosine kinase; RET-ELE1
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RET using anti-RET antibody (A00293-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates Lane 2: human HL-60 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RET antigen affinity purified polyclonal antibody (Catalog # A00293-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RET at approximately 170KD. The expected band size for RET is at 124KD.
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