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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

Anti-RFXANK Antibody

HPA053330
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetRFXANK
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RFXANK
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8625
  • Target name
    RFXANK
  • Target description
    regulatory factor X associated ankyrin containing protein
  • Target synonyms
    ANKRA1, BLS, F14150_1, MHC2D2, RFX-B, DNA-binding protein RFXANK, RFX-Bdelta4, ankyrin repeat family A protein 1, ankyrin repeat-containing regulatory factor X-associated protein, regulatory factor X subunit B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14593
  • Protein Name
    DNA-binding protein RFXANK
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Western Blot Positive WB detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate All lanes: RFXANK antibody at 6.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29, 25, 26 kDa Observed band size: 29 kDa
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Figure 1. Western blot analysis of RFXANK using anti-RFXANK antibody (A07934). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFXANK antigen affinity purified polyclonal antibody (Catalog # A07934) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFXANK at approximately 34 kDa. The expected band size for RFXANK is at 28 kDa.
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Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules and strong nuclear staining in cells in glomeruli.
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