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Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells, as well as positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells, as well as positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells, as well as positivity in neuropil.

Anti-RHOT1 Antibody

AMAB90852
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRHOT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RHOT1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL1083
  • Conjugate
    Unconjugated
  • Gene ID55288
  • Target name
    RHOT1
  • Target description
    ras homolog family member T1
  • Target synonyms
    ARHT1; MIRO1; MIRO-1; mitochondrial Rho (MIRO) GTPase 1; mitochondrial Rho GTPase 1; rac-GTP binding protein-like protein; ras homolog gene family, member T1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ8IXI2
  • Protein Name
    Mitochondrial Rho GTPase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MIRO1/RHOT1 using anti-MIRO1/RHOT1 antibody (A05928-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIRO1/RHOT1 antigen affinity purified polyclonal antibody (Catalog # A05928-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIRO1/RHOT1 at approximately 71 kDa. The expected band size for MIRO1/RHOT1 is at 71 kDa.
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