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Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

Anti-RIPK3 Antibody

HPA055087
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetRIPK3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RIPK3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11035
  • Target name
    RIPK3
  • Target description
    receptor interacting serine/threonine kinase 3
  • Target synonyms
    receptor interacting protein 3; receptor-interacting serine/threonine-protein kinase 3; RIP3; RIP-3; RIP-like protein kinase 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y572
  • Protein Name
    Receptor-interacting serine/threonine-protein kinase 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Phospho-RIP3 (S232) using anti-Phospho-RIP3 (S232) antibody (M00202S232). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse L929 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-RIP3 (S232) antigen affinity purified monoclonal antibody (Catalog # M00202S232) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Phospho-RIP3 (S232) at approximately 50 kDa. The expected band size for Phospho-RIP3 (S232) is at 50 kDa.
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