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Immunohistochemical staining of human colon shows cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows cytoplasmic and nuclear positivity in glandular cells.

Anti-RLIM Antibody

HPA018895
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRLIM
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RLIM
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51132
  • Target name
    RLIM
  • Target description
    ring finger protein, LIM domain interacting
  • Target synonyms
    E3 ubiquitin-protein ligase RLIM; E3 ubiquitin-protein ligase RNF12; LIM domain-interacting RING finger protein; MRX61; NY-REN-43; renal carcinoma antigen NY-REN-43; RING finger LIM domain-binding protein; ring finger protein 12; ring zinc finger LIM domain binding protein; ring zinc finger protein NY-REN-43antigen; RING-type E3 ubiquitin transferase RLIM; R-LIM; RNF12; TOKAS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NVW2
  • Protein Name
    E3 ubiquitin-protein ligase RLIM
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RLIM using anti-RLIM antibody (A05428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RLIM antigen affinity purified polyclonal antibody (Catalog # A05428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RLIM at approximately 69 kDa. The expected band size for RLIM is at 69 kDa.
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