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Mouse placenta lysates probed with Anti-RNF12 Polyclonal Antibody, Unconjugated (bs-9177R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C.
Mouse placenta lysates probed with Anti-RNF12 Polyclonal Antibody, Unconjugated (bs-9177R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C.
Mouse placenta lysates probed with Anti-RNF12 Polyclonal Antibody, Unconjugated (bs-9177R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C.

RNF12 Polyclonal Antibody

BS-9177R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetRLIM
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Overview

  • Supplier
    Bioss
  • Product Name
    RNF12 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IF(IHC-P)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID51132
  • Target name
    RLIM
  • Target description
    ring finger protein, LIM domain interacting
  • Target synonyms
    MRX61, NY-REN-43, RNF12, TOKAS, E3 ubiquitin-protein ligase RLIM, E3 ubiquitin-protein ligase RNF12, LIM domain-interacting RING finger protein, R-LIM, RING finger LIM domain-binding protein, RING-type E3 ubiquitin transferase RLIM, renal carcinoma antigen NY-REN-43, ring finger protein 12, ring zinc finger LIM domain binding protein, ring zinc finger protein NY-REN-43antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NVW2
  • Protein Name
    E3 ubiquitin-protein ligase RLIM
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RLIM using anti-RLIM antibody (A05428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RLIM antigen affinity purified polyclonal antibody (Catalog # A05428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RLIM at approximately 69 kDa. The expected band size for RLIM is at 69 kDa.
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