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ProductsBack/RLIM / RNF12 Antibody

RLIM / RNF12 Antibody

LS-C754112
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetRLIM
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RLIM / RNF12 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Applications Supplier
    ELISA, IP, WB Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51132
  • Target name
    RLIM
  • Target description
    ring finger protein, LIM domain interacting
  • Target synonyms
    E3 ubiquitin-protein ligase RLIM; E3 ubiquitin-protein ligase RNF12; LIM domain-interacting RING finger protein; MRX61; NY-REN-43; renal carcinoma antigen NY-REN-43; RING finger LIM domain-binding protein; ring finger protein 12; ring zinc finger LIM domain binding protein; ring zinc finger protein NY-REN-43antigen; RING-type E3 ubiquitin transferase RLIM; R-LIM; RNF12; TOKAS
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RLIM using anti-RLIM antibody (A05428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RLIM antigen affinity purified polyclonal antibody (Catalog # A05428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RLIM at approximately 69 kDa. The expected band size for RLIM is at 69 kDa.
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