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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in distal tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in distal tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in distal tubules.

Anti-RNASEH1 Antibody

HPA043037
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRNASEH1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RNASEH1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID246243
  • Target name
    RNASEH1
  • Target description
    ribonuclease H1
  • Target synonyms
    H1RNA, PEOB2, RNH1, ribonuclease H1, ribonuclease H type II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60930
  • Protein Name
    Ribonuclease H1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RNASEH1 using anti-RNASEH1 antibody (A06342-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNASEH1 antigen affinity purified polyclonal antibody (Catalog # A06342-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNASEH1 at approximately 38 kDa. The expected band size for RNASEH1 is at 32 kDa.
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