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Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.

Anti-RNF31 Antibody

HPA048745
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRNF31
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RNF31 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55072
  • Target name
    RNF31
  • Target description
    ring finger protein 31
  • Target synonyms
    HOIP, IMD115, Paul, ZIBRA, E3 ubiquitin-protein ligase RNF31, HOIL-1-interacting protein, RING-type E3 ubiquitin transferase RNF31, zinc in-between-RING-finger ubiquitin-associated domain protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96EP0
  • Protein Name
    E3 ubiquitin-protein ligase RNF31
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of RNF31 using anti-RNF31 antibody (A04457-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissues lysates Lane 2: human HepG2 whole cell lysates Lane 3: human PC-3 whole cell lysates Lane 4: human A549 whole cell lysates Lane 5: human U2OS whole cell lysates Lane 6: human Caco-2 whole cell lysates Lane 7: human Hela whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF31 antigen affinity purified polyclonal antibody (Catalog # A04457-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF31 at approximately 120KD. The expected band size for RNF31 is at 120KD.
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA856927LA01HU at dilution of 1:100
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