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RNF31 Antibody

LS-C749150
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetRNF31
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RNF31 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 57kDa/102kDa/119kDa, while the observed MW by Western blot was 110kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 57kDa/102kDa/119kDa, while the observed MW by Western blot was 110kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55072
  • Target name
    RNF31
  • Target description
    ring finger protein 31
  • Target synonyms
    HOIP, IMD115, Paul, ZIBRA, E3 ubiquitin-protein ligase RNF31, HOIL-1-interacting protein, RING-type E3 ubiquitin transferase RNF31, zinc in-between-RING-finger ubiquitin-associated domain protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNF31 using anti-RNF31 antibody (A04457-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissues lysates Lane 2: human HepG2 whole cell lysates Lane 3: human PC-3 whole cell lysates Lane 4: human A549 whole cell lysates Lane 5: human U2OS whole cell lysates Lane 6: human Caco-2 whole cell lysates Lane 7: human Hela whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF31 antigen affinity purified polyclonal antibody (Catalog # A04457-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF31 at approximately 120KD. The expected band size for RNF31 is at 120KD.
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