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Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts and Leydig cells.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts and Leydig cells.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts and Leydig cells.

Anti-RNF40 Antibody

HPA041330
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRNF40
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RNF40
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9810
  • Target name
    RNF40
  • Target description
    ring finger protein 40
  • Target synonyms
    BRE1B, RBP95, STARING, E3 ubiquitin-protein ligase BRE1B, 95 kDa retinoblastoma protein binding protein, 95 kDa retinoblastoma-associated protein, BRE1 E3 ubiquitin ligase homolog B, BRE1-B, RING-type E3 ubiquitin transferase BRE1B, Rb-associated protein, ring finger protein 40, E3 ubiquitin protein ligase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75150
  • Protein Name
    E3 ubiquitin-protein ligase BRE1B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RNF40 using anti-RNF40 antibody (A06979-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF40 antigen affinity purified polyclonal antibody (Catalog # A06979-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF40 at approximately 130 kDa. The expected band size for RNF40 is at 114 kDa.
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