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Anti-RNF43, Avi-Tag Antibody

102550
BPS Bioscience
ApplicationsOther Application
Product group Antibodies
TargetRNF43
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    Anti-RNF43, Avi-Tag Antibody
  • Delivery Days Customer
    23
  • Applications
    Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Estimated Purity
    ≥90%
  • Gene ID54894
  • Target name
    RNF43
  • Target description
    ring finger protein 43
  • Target synonyms
    E3 ubiquitin-protein ligase RNF43; RING-type E3 ubiquitin transferase RNF43; RNF124; SSPCS; URCC
  • Isotype
    IgG1
  • Protein IDQ68DV7
  • Protein Name
    E3 ubiquitin-protein ligase RNF43
  • Scientific Description
    This anti-RNF43 antibody is a purified, humanized recombinant monoclonal antibody that recognizes human RNF43 (ring finger protein 43). The heavy chain contains a C-terminal Avi-tag™. This antibody has been tested for binding activity in an ELISA assay.
  • Storage Instruction
    -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNF43 using anti-RNF43 antibody (A01694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF43 antigen affinity purified polyclonal antibody (Catalog # A01694-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF43 at approximately 86KD. The expected band size for RNF43 is at 86KD.
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IHC image of CSB-PA019892LA01HU diluted at 1:600 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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