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Immunohistochemical staining of human duodenum shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate nuclear positivity in glandular cells.

Anti-RNF43 Antibody

HPA008079
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRNF43
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RNF43
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54894
  • Target name
    RNF43
  • Target description
    ring finger protein 43
  • Target synonyms
    E3 ubiquitin-protein ligase RNF43; RING-type E3 ubiquitin transferase RNF43; RNF124; SSPCS; URCC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ68DV7
  • Protein Name
    E3 ubiquitin-protein ligase RNF43
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNF43 using anti-RNF43 antibody (A01694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF43 antigen affinity purified polyclonal antibody (Catalog # A01694-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF43 at approximately 86KD. The expected band size for RNF43 is at 86KD.
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