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Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells and decidual cells.
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells and decidual cells.
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells and decidual cells.

Anti-RNGTT Antibody

HPA003750
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRNGTT
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RNGTT
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8732
  • Target name
    RNGTT
  • Target description
    RNA guanylyltransferase and 5'-phosphatase
  • Target synonyms
    CAP1A; hCAP; HCAP1; HCE; HCE1; mRNA-capping enzyme
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60942
  • Protein Name
    mRNA-capping enzyme
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNGTT using anti-RNGTT antibody (A09317-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNGTT antigen affinity purified polyclonal antibody (Catalog # A09317-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNGTT at approximately 69 kDa. The expected band size for RNGTT is at 69 kDa.
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