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Western blot All lanes: RNGTT antibody at 1.42microg/ml + THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 69, 67, 53, 62 kDa Observed band size: 69 kDa
Western blot All lanes: RNGTT antibody at 1.42microg/ml + THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 69, 67, 53, 62 kDa Observed band size: 69 kDa
Western blot All lanes: RNGTT antibody at 1.42microg/ml + THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 69, 67, 53, 62 kDa Observed band size: 69 kDa

RNGTT Antibody

CSB-PA526449ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRNGTT
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Overview

  • Supplier
    Cusabio
  • Product Name
    RNGTT Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8732
  • Target name
    RNGTT
  • Target description
    RNA guanylyltransferase and 5'-phosphatase
  • Target synonyms
    CAP1A; hCAP; HCAP1; HCE; HCE1; mRNA-capping enzyme
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60942
  • Protein Name
    mRNA-capping enzyme
  • Scientific Description
    Bifunctional mRNA-capping enzyme exhibiting RNA 5-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5-diphosphate terminus.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNGTT using anti-RNGTT antibody (A09317-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNGTT antigen affinity purified polyclonal antibody (Catalog # A09317-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNGTT at approximately 69 kDa. The expected band size for RNGTT is at 69 kDa.
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