More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/HCE antibody [N1N3]

HCE antibody [N1N3]

GTX105694
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetRNGTT
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    HCE antibody [N1N3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.54 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8732
  • Target name
    RNGTT
  • Target description
    RNA guanylyltransferase and 5'-phosphatase
  • Target synonyms
    CAP1A, HCE, HCE1, hCAP, mRNA-capping enzyme, HCAP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60942
  • Protein Name
    mRNA-capping enzyme
  • Scientific Description
    Bifunctional mRNA-capping enzyme exhibiting RNA 5-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5-diphosphate terminus.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Diamant G, Amir-Zilberstein L, Yamaguchi Y, et al. DSIF restricts NF-κB signaling by coordinating elongation with mRNA processing of negative feedback genes. Cell Rep. 2012,2(4):722-31. doi: 10.1016/j.celrep.2012.08.041
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
RNGTT AntibodyCAB6431
ReactivityHuman
TargetRNGTT
  • SizePrice
Anti-RNGTT Antibody
Product group Antibodies
Antibodies.com
Anti-RNGTT AntibodyA31377
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
RayBiotech
Anti-RNGTT Antibody144-06431
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetRNGTT
  • SizePrice
WB analysis of various sample lysates using GTX54686 HCE antibody. Dilution : 1:1000 Loading : 25microg per lane
Product group Antibodies
GeneTex
HCE antibodyGTX54686
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetRNGTT
  • SizePrice
Product group Antibodies
MedChem Express
HCE Antibody (YA2959, Rabbit)HY-P83214
TargetRNGTT
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
RNGTT / HCAP AntibodyLS-C335668
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRNGTT
  • SizePrice
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells and decidual cells.
Product group Antibodies
Atlas Antibodies
Anti-RNGTT AntibodyHPA003750
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRNGTT
  • SizePrice
Western blot All lanes: RNGTT antibody at 1.42microg/ml + THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 69, 67, 53, 62 kDa Observed band size: 69 kDa
Product group Antibodies
Cusabio
RNGTT AntibodyCSB-PA526449ESR2HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetRNGTT
  • SizePrice
Figure 1. Western blot analysis of RNGTT using anti-RNGTT antibody (A09317-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNGTT antigen affinity purified polyclonal antibody (Catalog # A09317-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNGTT at approximately 69 kDa. The expected band size for RNGTT is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-RNGTT Antibody Picoband(r)A09317-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetRNGTT
  • SizePrice