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Anti-ROBO-1 [B2212A]

Ab00398-1.1
Absolute Antibody
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetROBO1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-ROBO-1 [B2212A]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody binds to human ROBO-1, a receptor for SLIT1 and SLIT2. ROBO-1 is involved in axonal navigation, lung development, and cell migration.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    B2212A
  • Gene ID6091
  • Target name
    ROBO1
  • Target description
    roundabout guidance receptor 1
  • Target synonyms
    deleted in U twenty twenty; DUTT1; roundabout homolog 1; roundabout, axon guidance receptor, homolog 1; SAX3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9Y6N7
  • Protein Name
    Roundabout homolog 1
  • Scientific Description
    This full-length, reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Human recombinant ROBO-1.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROBO1 at approximately 250 kDa. The expected band size for ROBO1 is at 181 kDa.
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