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Western Blot Positive WB detected in: Hela whole cell lysate All lanes: ROBO1 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 181, 182, 177, 176, 170 kDa Observed band size: 181 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: ROBO1 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 181, 182, 177, 176, 170 kDa Observed band size: 181 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: ROBO1 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 181, 182, 177, 176, 170 kDa Observed band size: 181 kDa

ROBO1 Antibody

CSB-PA896760LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetROBO1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ROBO1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6091
  • Target name
    ROBO1
  • Target description
    roundabout guidance receptor 1
  • Target synonyms
    deleted in U twenty twenty; DUTT1; roundabout homolog 1; roundabout, axon guidance receptor, homolog 1; SAX3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6N7
  • Protein Name
    Roundabout homolog 1
  • Scientific Description
    Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10102268, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed:26529257). May be required for lung development (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROBO1 at approximately 250 kDa. The expected band size for ROBO1 is at 181 kDa.
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